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mntR [2019-11-11 11:25:26]
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mntR [2019-11-11 11:25:26]

transcriptional regulator, controls manganese import and efflux
locus
BSU_24520
pI
5.63
mw
16.60 kDa
protein length
142 aa Sequence Blast
gene length
429 bp Sequence Blast
function
regulation of manganese transport
product
transcriptional regulator (DtxR family)
essential
no
synonyms
yqhN
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.2|Manganese]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

    • Gene

    Coordinates
    2,543,440 → 2,543,868

    Phenotypes of a mutant

  • inactivation of ''[gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]'' reduces sporulation efficiency to 8.9% that of wild type cells [Pubmed|26735940]
  • highly sensitive to Mn(II) intoxication [Pubmed|27748968]

    • The protein

    Protein family

  • DtxR/MntR family (single member, according to UniProt)

    • [SW|Domains]

  • HTH dtxR-type domain (aa 1-63) (according to UniProt)

    • [SW|Cofactors]

  • Mn(2+) acts as co-repressor (according to [Pubmed|20408793])

    • Structure

  • [PDB|2F5D] (complex with manganese), [PDB|2HYG] (apo-form)

    • additional information

  • information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000054 PRODORIC2 database]

    • Expression and Regulation

    Operons

    genes
    [gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]
    description
    [pubmed|22383849]
    view in new tab

    genes
    [gene|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|lipM]-[gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-C435 (yqhN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2433 NBRP B. subtilis, Japan]
  • BKE24520 (Δ[gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGAAACCCTCCCAAAAA, downstream forward: _UP4_TAAAAAGGCGGTCTCGAAGG
  • BKK24520 (Δ[gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGAAACCCTCCCAAAAA, downstream forward: _UP4_TAAAAAGGCGGTCTCGAAGG

    • labs

  • [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
  • [SW|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]

    • References

    Reviews

  • 20408793,25160631

    • Original Publications

  • 17176058,12675807,17118401,16533030,15736948,14580210,12847518,23298157,12950915,10760146,26735940,27748968